The appropriate data was then pasted into a graphpad prism spreadsheet for linear regression analysis and for determining mean c t values. In this study the data was analyzed statistically by using spss version 16 wayne and daniel 1995 roger et al 2003. It is imperative that all users read the genexpert. The ability of the portable cepheid smartcycler realtime pcr machine to detect footandmouth disease fmd virus sensitively and accurately was evaluated by comparing the results of the analyses of nasal swab and serum samples from experimentally infected animals with those obtained from the realtime pcr assay currently in use in the laboratory. Cepheid smartcycler system software license agreement. If the share price of a security gets too high, a company can perform a stock split by issuing all shareholders an extra share, thereby halving the price of an individual share.
Cepheid xpert mrsa assay package insert pi contains system performance data and describes testing principle and procedure, qc recommendations, and limitations. By automating highly complex and timeconsuming manual procedures, the companys solutions deliver a better way for institutions of any size to perform sophisticated genetic testing for organisms and geneticbased. It seems to be working well, but no assays have actually been performed using the. In 2010, a total of 10 stations that were sampled in 2009 were revisited. Smart cycler ii can mimic the conditions of other realtime cyclers. Pcr, cepheid smart cycler was applied by using qiaamp mini column kit and sacace hcv genotyping kitto estimate the viral load and to investigate the genotypes of hcv. Every icore module con tains a highly thermal conductive ceramic plate, a fan and two small optical blocks. Detection of food pathogens using the smart cycler ii. All samples berlin and st etienne were shipped at 20 c to leeds for testing with an inhouse gdh realtime pcr assay.
The internal quality controls included in each cepheid cartridge check every step of the assay for every patient specimen, not just once a day. Available realtime rtpcr cycler and kinetic pcr machines. Cepheids genexpert system is the worlds most flexible platform, providing healthcare professionals with a best in class test menu for any setting from the core lab to near patient applications. Prevalence and associated risk factors of hepatitis c. Sas programs for realtime rtpcr having multiple independent. Rotorgene corbett research, australia, iq icycler iq biorad, usa, mx mx3000 stratagene,usa, sc smart cycler cepheid, usa. Manufacturer specifications smartcycler ii td, cepheid please note. Freezer, biomerieux nuclisens easymag system with software version 2. Cepheid smartcycler system software license agreement license grant. Attantion number of tests includes controls and calibrators for quantitative tests 5 pcr diagnostics kits sexually transmitted infections chlamydia trachomatis packaging arrangement. Stock splits are used by cepheid to keep share prices within reasonable numbers to encourage investment. As cepheid is set into motion by its spring and magnetic escapement mechanism, it creates a unique visual display of dancing shapes and mesmerizing patterns.
Briefly, samples were diluted in star buffer roche. Each instrument contains 16 independently programmable modules that can perform four color, realtime fluorometric detection. One of the pdf files decribes a comparison between lightcycler, smartcycler and abi on sensitivity. Acquired cepheid smart cycler using county cri and phep grant funds increase public health capacity for molecular testing ability to accept routine testing as overflow site trialed us epa taqman entercoccus assay pre method a vs. Accurate and statistically verified quantification of. As an early example, following the attack on september 11, 2001, cepheid was awarded. Users should thoroughly read the method in its entirety before preparation of reagents and commencement of the method to identify differences in protocols for different platforms. Ala14, biosan biosan eph electrophoresis in agarose gel. The paper from raja raja et al clinical chemistry 48. Evaluation of cepheid smartcycler and euroclone duplicareal time. Cepheid smartcycler emission spectra of cal fluor and quasar dyes. Evaluation of the smartcycler ii system for realtime detection of. Cphd, california, us was used for the amplification process. The cepheid smart cycler ii system is an integrated dnarna amplification and detection instrument based on the microprocessorcontrolled.
Results and discussion three hundred blood samples were screened for the presence of anti hcv antibodies. Among them, the simplexa bordetella pertussisparapertussis assay focus diagnostics, the smartcycler bordetella pertussisparapertussis assay cepheid, and bordetella rgene argene. Daily use of external controls provides no additional. Acanthamoeba keratitis ak infection needs to be diagnosed definitively to optimize therapy in order to avoid possible visual impairment. To shut down the smartcycler software, choose exit from the file menu, or click the. Parameters involved in the conversion of realtime pcr assays. Evaluation of four commercial realtime pcr assays for. We have written a visual basic excel macro that facilitates determination and conversion of the appropriate subset of smart cycler table 1. The assay was performed with the cepheid sunnyvale, calif. Find out why cepheid s solutions are a top choice for fast, accurate, and easy diagnostic testing. Used cepheid genexpert iv thermal cycler for sale dotmed. Jun 06, 2018 optics data was exported from the cepheid smart cycler as comma separated values files. Using quantification cycle values extracted from qpcr instruments, it uses a proven and universally applicable model to give finalized quantification resu. It is the responsibility of employees to ensure that they are using the correct revision of this document by checking the document revision level with that held on the i drive, folder iso.
Cepheid smartcycler ii real time instrument with dx software version 1. Cepheid smartcycler ii td pcr manufacturer specifications. Fully automated detection of mrsa from nasal swab appropriate collection of nasal swab is very important smart cycler. This random access instrument features 16 reaction sites, each of which can be run with a different protocol. Method 1611 describes a quantitative polymerase chain reaction qpcr procedure for the detection of dna from enterococci bacteria in ambient water matrices based on the amplification and detection of. Leinster acknowledged that he brought out circumstances owners guide pdf relating to windoms demeanor at the time of the. The user should refer to the platform specific instructions for these instruments in the appendices. Evaluation of cepheid smartcycler and euroclone duplicareal time dual easy ct assay for confirmation of chlamydia. Installation of the smartcycler system is plugandplay, and system expansion only requires an additional usb connection. Warnings and precautions treat all specimensamples as potentially infectious. Smart cycler ii system, capable of performing cycling parameters described in this sop and simultaneous realtime detection for fam, tet, texas red and cy5 fluorophores, cepheid. Pdf the ability of the portable cepheid smartcycler realtime pcr machine to detect footandmouth disease fmd virus sensitively and. Fastfinder platform for automated pcranalysis healthtech event eindhoven 24112015.
Parameters involved in the conversion of realtime pcr assays from the abi prism 7700 to the cepheid smartcycler ii. Read the complete quick reference instructions before performing the test. Workquality assurance project plan njdep new jersey. The kit contains sufficient reagents, in lyophilized bead form, to qualitatively assay 50 clinical samples for both leishmania genus and l. L, minicentrifuge with adapter for cepheid reaction tubes, cepheid cooling block, u. Cepheid smart cycler fam, atto 488 cy3, tet, atto 550, tamra texas red, rox, lc 610 cy5, atto 647n, dy649 4plex mx3000p fam, atto 488 tet, atto 520 hex, joe, yakima yellow cy3, atto 550, dy569 tamra, atto 565.
With an average timetoresults of 2040 minutes, the smartcycler system can deliver. Click on the smart cycler icon to start the life science 2d smartcycler software. Upload files that contain software or other material protected by intellectual property laws or by rights of privacy of publicity unless you own or control the rights thereto or have received all necessary consents. The following outlines a rapid and robust method for detecting the human multidrug resistance gene mdr1 snp c3435t using real time polymerase chain reaction pcr. Pdf evaluation of the portable cepheid smartcycler realtime. Store the xpert xpress sarscov2 cartridges at 228 c. Cepheid is a wooden kinetic sculpture named after pulsating stars.
We evaluated the performances of 4 commercial realtime pcr kits for bordetella pertussis is481 sequence detection in nasopharyngeal aspirates by comparison with an inhouse realtime pcr assay. In the thresh setting column, enter manual threshold for each channel influenza a. This pi is located in the microbiology laboratorys pi files. Brecher, va boston health care system broadcast live from apic c. The smarttube optical windows are set at 90 degree angles for the ideal optical read. The optical system of the unit is centered around the icore, a solid state module which does not require warm up, maintenance, or normalization dye. Plate from the file menu, designate the plate type as background, and click ok. Upload files that contain viruses, corrupted files, or any other similar software or. Office of water 4303t 1200 pennsylvania avenue, nw.
A positive control with ms2 rna and a negative control of pcr water was included in each run. Wet lab smartcycler wetlab2 research on stationresearch on. The primary outcome of interest was the presence and frequency of ms2 andor fluorescent. No specific limitations were noted in the pi that would affect. Researchers can receive results within 24 hours of. Comparison of smartcycler realtime reverse transcriptionpcr. For analysis by the standard curve method of pfaffl 2001, c t data was exported from the cepheid smart cycler as comma separated values files. Find out why cepheids solutions are a top choice for fast, accurate, and easy diagnostic testing. The correlation between rtpcr and elisa assay on hepatitis c. Fast with the smartcycler system, you can optimize pcr denaturation, annealing and extension temperatures and times in a single run. For assistance, call cepheid technical support at 888 838 3222. United states department of agriculture agricultural. None of the equipment posted for sale on is owned by medwow, should you have any questions regarding a specific item, please direct them to the appropriate seller by making use of the available communication channels on the items page. Comparison of smartcycler realtime reverse transcriptionpcr assay in a.
The university of michigan has the listed equipment for sale on a bid basis. We have written a visual basic excel macro that facilitates determination and conversion of the appropriate subset of smart cycler optics data to a logarithmic format for subsequent analysis. Computer is windows 2000 with cepheid smart cycler version 2. The thin especiallyformed reaction vessels made of polypropylene are pushed into a small slot to establish contact with the ce. Consequently, a collaborative project to establish a smartcycler rtpcr for influenza a. Incompatibility of detection format and instrument. Cepheid s genexpert system is the worlds most flexible platform, providing healthcare professionals with a best in class test menu for any setting from the core lab to near patient applications. Our hours of operation are monday through friday, 8. Pcr diagnostics kits extraction kits qualichem sea.
The cycle threshold for all positive results was recorded. Customer contacts cepheid to order an xpert calibration kit before the end of the calibration period. Cepheid is a leading molecular diagnostics company dedicated to improving healthcare by developing, manufacturing, and marketing accurate yet easytouse molecular systems and tests. Pdf evaluation of the portable cepheid smartcycler real. Roches thermal cycler takes 30 minutes for 40 cycles, and qiagens about 40 minutes for 40 cycles.
These samples were collected from the patients who have visited the hospital purposely. The smarttube optical windows are set at 90 degree angles for the ideal optical read path. The smartcycler dx system is a rapid, realtime thermal cycler used for identifying dna or rna from prepared biological samples. Kits chemistries adapted to slow ramp rates can be used in the smart cycler ii martina fricker qpcr 2005 07. Smart cycler cepheid, dt96 dnatechnology fep fluorescent endpoint detection. Problems with fast ramp rate chemistry not compatible. University of michigan property disposition medical equipment bid june 2017. During pcr cycling, optical signals from two sequence specific fluorescent probes within the reaction are monitored in real time. However, the thermal mass and conductivity of the air still cause some delay 10. Click show analysis and the wells depicted in the plate layout will turn black. Cepheid has used an icore ceramic heating plate in its smart cycler, heating samples in disposable tubes and. University of michigan property disposition medical equipment. Subject to the terms and conditions herein, cepheid grants customer a nonexclusive, nontransferable license the license to use one 1 copy of the cepheid smart cycler system software program the software on the computer provided with the smart. Cepheid enabling access to molecular diagnostic testing.
Calibration required after 1 year or 2,000 runsmodule cepheid ships replacement module to customers onsite intervention required to replace modules. In this process, the probe anneals specifically to the template followed by primer extension and amplification. This procedure allows the instrument to measure the background signal of each well. Wear suitable protective clothing, gloves, and eyeface protection when handling the. Cepheid products based on llnl technology include the smart cycler and genexpert instruments which rapidly perform a broad range of genetic tests, from identifying infectious organisms to evaluating atrisk populations for the early detection of disease. Objectiveto compare the smartcycler ii system cepheid, sunnyvale, calif results. Maintenance of genexpert fondazione centro san raffaele.
For the qualitative detection and identification of respiratory syncytial virus and human metapneumovirus viral rna extracted from nasal swab and nasopharyngeal swab specimens. Its curvy, raylike forms slice and meld into each other as they spin and rock in a seemingly random motion. On this page the most prominent realtime pcr cycler are described. The smartcycler protocol offers a single, closedtube format, which prevents. University of michigan property disposition medical equipment bid list. Automated diagnostic results multiple input one output roche lightcycler applied biosystems 7500. Cepheid smart cycler with quantitect probe rtpcr kit qiagen, valencia, ca. Pcr primers used in this study gene primer sequence 18s forward 5. Methods genomic dna was extracted from whole blood. View and download genexpert xpert mtb rif user manual online. Within the calibrate submenu, select extract background component. Manual extraction, but automated amplification and detection bd max.
The smart leish consists of an assay reagent kit and assay definition files for the polymerase chain reaction pcr instrument platform. Cepheid smartcycler ii thermal cycler automated realtime. Evaluation of the portable cepheid smartcycler realtime. Manufacturer cepheid 904 caribbean drive sunnyvale, ca 94089 united states. Transferring assays between different platforms genequantification. The cepheid smartcycler ii is a modular real time pcr system. Subject to the terms and conditions herein, cepheid grants customer a nonexclusive, nontransferable license the license to use one 1 copy of the cepheid smartcycler system software program the software on the computer provided with the smart. Cepheid smart cycler ii rtpcr model sc2 with 16 well block, including computer with software. Method 1600 composite sample analysis north beach 4 transects. Each county sampled five sites every other week over a nine week period. Evaluation of the portable cepheid smartcycler realtime pcr machine for the rapid diagnosis of footandmouth disease article pdf available in the veterinary record 15020. A series of studies were preformed evaluating a number of routinely used viral transport medium at a volume of 2 ml.
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